one step primescript q-pcr kit  (Tokyo Chemical Industry)

Journal: World Journal of Surgical Oncology

Article Title: Identification of critical prognosis signature associated with lymph node metastasis of stomach adenocarcinomas

doi: 10.1186/s12957-023-02940-y

Figure Lengend Snippet: Expression validation of ten-mRNA prognostic features based on LNM-DEGs. A q RT-PCR validation of 10-mRNA expression levels in gastric cancer cell lines (AGS and MGC-803) and normal cell lines (GES-1). B Immunohistochemical map of protein expression in STAD and normal gastric tissues. Data were obtained from the Human Protein Atlas online database. ** P < 0.01 and * P < 0.05

Article Snippet: The total RNA was extracted using a Trizol reagent (Invitrogen, CN). cDNA was then synthesized using the Advantage RT-for-PCR Kit (Clontech), diluted, and subjected to q RT-PCR using the HiScript® II One Step q RT-PCR SYBR ® Green Kit (Takara, Japan). q RT-PCR was performed (Table S ).

Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Immunohistochemical staining